A Novel Chromodomain Protein, Pdd3p, Associates with Internal Eliminated Sequences during Macronuclear Development in Tetrahymena thermophila
نویسندگان
چکیده
منابع مشابه
Macronuclear Persistence of Sequences Normally Eliminated During Development in Tetrahymena thermophila
During conjugation in the ciliated protozoan, Tetrahymena thermophilu , a somatic MACronucleus develops from the germinal MICronucleus. Ten to 20 percent of the MIC genome is eliminated during this process. Three repetitive families have been identified which have different levels of repetition in the MIC and are eliminated to different degrees in the MAC. Some members of two of these families ...
متن کاملAlternative processing of sequences during macronuclear development in Tetrahymena thermophila.
DNA is eliminated during development of the somatic MACronucleus from the germinal MICronucleus in the ciliated protozoan, Tetrahymena thermophila. Facultatively persistent sequences are a class of sequences that persist in the MAC DNA of some cell lines but are eliminated from the MAC DNA of other cell lines. One cloned MAC fragment contains a persistent sequence as well as sequences normally ...
متن کاملRole of micronucleus-limited DNA in programmed deletion of mse2.9 during macronuclear development of Tetrahymena thermophila.
Extensive programmed DNA rearrangements occur during the development of the somatic macronucleus from the germ line micronucleus in the sexual cycle of the ciliated protozoan Tetrahymena thermophila. Using an in vivo processing assay, we analyzed the role of micronucleus-limited DNA during the programmed deletion of mse2.9, an internal eliminated sequence (IES). We identified a 200-bp region wi...
متن کاملHigh frequency intragenic recombination during macronuclear development in Tetrahymena thermophila restores the wild-type SerH1 gene.
Macronuclear development in ciliates is characterized by extensive rearrangement of genetic material, including sequence elimination, chromosome fragmentation and telomere addition. Intragenic recombination is a relatively rare, but evolutionarily important phenomenon occurring in mitosis and meiosis in a wide variety of organisms. Here, we show that high frequency intragenic recombination, on ...
متن کاملChromodomain protein Tcd1 is required for macronuclear genome rearrangement and repair in Tetrahymena
The survival of an organism's progeny depends on the maintenance of its genome. Programmed DNA rearrangement and repair in Tetrahymena occur during the differentiation of the developing somatic macronuclear genome from the germ line micronuclear genome. Tetrahymena chromodomain protein (Tcd1) exhibited dynamic localization from the parental to the developing macronuclei. In the developing macro...
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ژورنال
عنوان ژورنال: Molecular and Cellular Biology
سال: 2000
ISSN: 0270-7306,1098-5549
DOI: 10.1128/mcb.20.11.4128-4134.2000